AustralieFrV1, Main, Exploration, bibRecord, 005897

Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group

Identifieur interne : 005897 ( Main/Exploration ); précédent : 005896; suivant : 005898

Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group

Auteurs : T. M. Westers [Pays-Bas] ; R. Ireland [Royaume-Uni] ; W. Kern [Allemagne] ; C. Alhan [Pays-Bas] ; J. S. Balleisen [Allemagne] ; P. Bettelheim [Autriche] ; K. Burbury [Australie] ; M. Cullen [Royaume-Uni] ; J. A. Cutler [États-Unis] ; M. G. Della Porta [Italie] ; A. M. Dr Ger [Pays-Bas] ; J. Feuillard [France] ; P. Font [Espagne] ; U. Germing [Allemagne] ; D. Haase [Allemagne] ; U. Johansson [Royaume-Uni] ; S. Kordasti [Royaume-Uni] ; M. R. Loken [États-Unis] ; L. Malcovati [Italie] ; J. G. Te Marvelde [Pays-Bas] ; S. Matarraz [Espagne] ; T. Milne [Royaume-Uni] ; B. Moshaver [Pays-Bas] ; G. J. Mufti [Royaume-Uni] ; K. Ogata [Japon] ; A. Orfao [Espagne] ; A. Porwit [Suède] ; K. Psarra [Grèce] ; S. J. Richards [Royaume-Uni] ; D. Subira [Espagne] ; V. Tindell [Royaume-Uni] ; T. Vallespi [Espagne] ; P. Valent [Autriche] ; Vhj Van Der Velden [Pays-Bas] ; T. M. De Witte [Pays-Bas] ; D. A. Wells [États-Unis] ; F. Zettl [Allemagne] ; M. C. Bene [France] ; Aa Van De Loosdrecht [Pays-Bas]

Source :

Leukemia [ 0887-6924 ] ; 2012.

RBID : Pascal:12-0303376

Descripteurs français

Pascal (Inist)
- Syndrome myélodysplasique, Normalisation, Cytométrie flux, International, Europe, Consensus, Hématologie.
Wicri :
- topic : Normalisation.

English descriptors

KwdEn :
- Consensus, Europe, Flow cytometry, Hematology, International, Myelodysplastic syndrome, Standardization.

Abstract

Flow cytometry (FC) is increasingly recognized as an important tool in the diagnosis and prognosis of myelodysplastic syndromes (MDS). However, validation of current assays and agreement upon the techniques are prerequisites for its widespread acceptance and application in clinical practice. Therefore, a working group was initiated (Amsterdam, 2008) to discuss and propose standards for FC in MDS. In 2009 and 2010, representatives from 23, mainly European, institutes participated in the second and third European LeukemiaNet (ELN) MDS workshops. In the present report, minimal requirements to analyze dysplasia are refined. The proposed core markers should enable a categorization of FC results in cytopenic patients as 'normal', 'suggestive of', or 'diagnostic of' MDS. An FC report should include a description of validated FC abnormalities such as aberrant marker expression on myeloid progenitors and, furthermore, dysgranulopoiesis and/or dysmonocytopoiesis, if at least two abnormalities are evidenced. The working group is dedicated to initiate further studies to establish robust diagnostic and prognostic FC panels in MDS. An ultimate goal is to refine and improve diagnosis and prognostic scoring systems. Finally, the working group stresses that FC should be part of an integrated diagnosis rather than a separate technique.

Affiliations:

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Le document en format XML

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<s3>ESP</s3>
<sZ>32 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Valent, P" sort="Valent, P" uniqKey="Valent P" first="P." last="Valent">P. Valent</name>
<affiliation wicri:level="3"><inist:fA14 i1="22"><s1>Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna</s1>
<s2>Vienna</s2>
<s3>AUT</s3>
<sZ>33 aut.</sZ>
</inist:fA14>
<country>Autriche</country>
<placeName><settlement type="city">Vienne (Autriche)</settlement>
<region nuts="2" type="province">Vienne (Autriche)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Der Velden, Vhj Van" sort="Der Velden, Vhj Van" uniqKey="Der Velden V" first="Vhj Van" last="Der Velden">Vhj Van Der Velden</name>
<affiliation wicri:level="3"><inist:fA14 i1="14"><s1>Department of Immunology, Erasmus MC</s1>
<s2>Rotterdam</s2>
<s3>NLD</s3>
<sZ>20 aut.</sZ>
<sZ>34 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Rotterdam</settlement>
<region nuts="2" type="province">Hollande-Méridionale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Witte, T M" sort="De Witte, T M" uniqKey="De Witte T" first="T. M." last="De Witte">T. M. De Witte</name>
<affiliation wicri:level="3"><inist:fA14 i1="16"><s1>Department of Hematology, St Radboud University Medical Center</s1>
<s2>Nijmegen</s2>
<s3>NLD</s3>
<sZ>23 aut.</sZ>
<sZ>35 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Nimègue</settlement>
<region type="province" nuts="2">Gueldre</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wells, D A" sort="Wells, D A" uniqKey="Wells D" first="D. A." last="Wells">D. A. Wells</name>
<affiliation wicri:level="1"><inist:fA14 i1="08"><s1>HematoLogics, Inc.</s1>
<s2>Seattle, WA</s2>
<s3>USA</s3>
<sZ>9 aut.</sZ>
<sZ>18 aut.</sZ>
<sZ>36 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Seattle, WA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zettl, F" sort="Zettl, F" uniqKey="Zettl F" first="F." last="Zettl">F. Zettl</name>
<affiliation wicri:level="3"><inist:fA14 i1="12"><s1>Department of Hematology and Oncology, Georg-August-University</s1>
<s2>Göttingen</s2>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
<sZ>37 aut.</sZ>
</inist:fA14>
<country>Allemagne</country>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Göttingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bene, M C" sort="Bene, M C" uniqKey="Bene M" first="M. C." last="Bene">M. C. Bene</name>
<affiliation wicri:level="4"><inist:fA14 i1="23"><s1>Faculty of Medicine and CHU, Nancy Université</s1>
<s2>Nancy</s2>
<s3>FRA</s3>
<sZ>38 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Grand Est</region>
<region type="old region">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
<settlement type="city">Nancy</settlement>
</placeName>
<orgName type="university">Nancy-Université</orgName>
</affiliation>
</author>
<author><name sortKey="De Loosdrecht, Aa Van" sort="De Loosdrecht, Aa Van" uniqKey="De Loosdrecht A" first="Aa Van" last="De Loosdrecht">Aa Van De Loosdrecht</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Hematology, VU University Medical Center</s1>
<s2>Amsterdam</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>39 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Amsterdam</settlement>
<region nuts="2" type="province">Hollande-Septentrionale</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">12-0303376</idno>
<date when="2012">2012</date>
<idno type="stanalyst">PASCAL 12-0303376 INIST</idno>
<idno type="RBID">Pascal:12-0303376</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">001228</idno>
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<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000D47</idno>
<idno type="wicri:doubleKey">0887-6924:2012:Westers T:standardization:of:flow</idno>
<idno type="wicri:Area/Main/Merge">005B82</idno>
<idno type="wicri:Area/Main/Curation">005897</idno>
<idno type="wicri:Area/Main/Exploration">005897</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group</title>
<author><name sortKey="Westers, T M" sort="Westers, T M" uniqKey="Westers T" first="T. M." last="Westers">T. M. Westers</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Hematology, VU University Medical Center</s1>
<s2>Amsterdam</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>39 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Amsterdam</settlement>
<region nuts="2" type="province">Hollande-Septentrionale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ireland, R" sort="Ireland, R" uniqKey="Ireland R" first="R." last="Ireland">R. Ireland</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Haematology, King's College Hospital</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>2 aut.</sZ>
<sZ>17 aut.</sZ>
<sZ>22 aut.</sZ>
<sZ>24 aut.</sZ>
<sZ>31 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kern, W" sort="Kern, W" uniqKey="Kern W" first="W." last="Kern">W. Kern</name>
<affiliation wicri:level="3"><inist:fA14 i1="03"><s1>MLL Munich Leukemia Laboratory</s1>
<s2>Munich</s2>
<s3>DEU</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
<country>Allemagne</country>
<placeName><region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
<settlement type="city">Munich</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Alhan, C" sort="Alhan, C" uniqKey="Alhan C" first="C." last="Alhan">C. Alhan</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Hematology, VU University Medical Center</s1>
<s2>Amsterdam</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>39 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Amsterdam</settlement>
<region nuts="2" type="province">Hollande-Septentrionale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Balleisen, J S" sort="Balleisen, J S" uniqKey="Balleisen J" first="J. S." last="Balleisen">J. S. Balleisen</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Department of Hematology Oncology and Clinical Immunology, Heinrich-Heine-University</s1>
<s2>Düsseldorf</s2>
<s3>DEU</s3>
<sZ>5 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
<country>Allemagne</country>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Düsseldorf</region>
<settlement type="city">Düsseldorf</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bettelheim, P" sort="Bettelheim, P" uniqKey="Bettelheim P" first="P." last="Bettelheim">P. Bettelheim</name>
<affiliation wicri:level="1"><inist:fA14 i1="05"><s1>First Medical Department, Elisabethinen Hospital</s1>
<s2>Linz</s2>
<s3>AUT</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Autriche</country>
<wicri:noRegion>Linz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Burbury, K" sort="Burbury, K" uniqKey="Burbury K" first="K." last="Burbury">K. Burbury</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Hematology, Peter MacCallum Cancer Centre</s1>
<s2>Melbourne, Victoria</s2>
<s3>AUS</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Melbourne, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cullen, M" sort="Cullen, M" uniqKey="Cullen M" first="M." last="Cullen">M. Cullen</name>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Haematology, HMDS, St James's University Hospital</s1>
<s2>Leeds</s2>
<s3>GBR</s3>
<sZ>8 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<wicri:noRegion>Leeds</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cutler, J A" sort="Cutler, J A" uniqKey="Cutler J" first="J. A." last="Cutler">J. A. Cutler</name>
<affiliation wicri:level="1"><inist:fA14 i1="08"><s1>HematoLogics, Inc.</s1>
<s2>Seattle, WA</s2>
<s3>USA</s3>
<sZ>9 aut.</sZ>
<sZ>18 aut.</sZ>
<sZ>36 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Seattle, WA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Della Porta, M G" sort="Della Porta, M G" uniqKey="Della Porta M" first="M. G." last="Della Porta">M. G. Della Porta</name>
<affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Department of Hematology and Oncology, Fondazione IRCCS Policlinico San Matteo and University of Pavia</s1>
<s2>Pavia</s2>
<s3>ITA</s3>
<sZ>10 aut.</sZ>
<sZ>19 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dr Ger, A M" sort="Dr Ger, A M" uniqKey="Dr Ger A" first="A. M." last="Dr Ger">A. M. Dr Ger</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Hematology, VU University Medical Center</s1>
<s2>Amsterdam</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>39 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Amsterdam</settlement>
<region nuts="2" type="province">Hollande-Septentrionale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Feuillard, J" sort="Feuillard, J" uniqKey="Feuillard J" first="J." last="Feuillard">J. Feuillard</name>
<affiliation wicri:level="3"><inist:fA14 i1="10"><s1>Laboratoire d'Hématologie, CHU Dupuytren</s1>
<s2>Limoges</s2>
<s3>FRA</s3>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Nouvelle-Aquitaine</region>
<region type="old region">Limousin</region>
<settlement type="city">Limoges</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Font, P" sort="Font, P" uniqKey="Font P" first="P." last="Font">P. Font</name>
<affiliation wicri:level="3"><inist:fA14 i1="11"><s1>Department of Hematology, Hospital General Universitario Gregorio Marañón</s1>
<s2>Madrid</s2>
<s3>ESP</s3>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<placeName><settlement type="city">Madrid</settlement>
<region nuts="2" type="region">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Germing, U" sort="Germing, U" uniqKey="Germing U" first="U." last="Germing">U. Germing</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Department of Hematology Oncology and Clinical Immunology, Heinrich-Heine-University</s1>
<s2>Düsseldorf</s2>
<s3>DEU</s3>
<sZ>5 aut.</sZ>
<sZ>14 aut.</sZ>
</inist:fA14>
<country>Allemagne</country>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Düsseldorf</region>
<settlement type="city">Düsseldorf</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Haase, D" sort="Haase, D" uniqKey="Haase D" first="D." last="Haase">D. Haase</name>
<affiliation wicri:level="3"><inist:fA14 i1="12"><s1>Department of Hematology and Oncology, Georg-August-University</s1>
<s2>Göttingen</s2>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
<sZ>37 aut.</sZ>
</inist:fA14>
<country>Allemagne</country>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Göttingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Johansson, U" sort="Johansson, U" uniqKey="Johansson U" first="U." last="Johansson">U. Johansson</name>
<affiliation wicri:level="1"><inist:fA14 i1="13"><s1>Department of Haematology, University Hospitals NHS Foundation Trust</s1>
<s2>Bristol</s2>
<s3>GBR</s3>
<sZ>16 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<wicri:noRegion>Bristol</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kordasti, S" sort="Kordasti, S" uniqKey="Kordasti S" first="S." last="Kordasti">S. Kordasti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Haematology, King's College Hospital</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>2 aut.</sZ>
<sZ>17 aut.</sZ>
<sZ>22 aut.</sZ>
<sZ>24 aut.</sZ>
<sZ>31 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Loken, M R" sort="Loken, M R" uniqKey="Loken M" first="M. R." last="Loken">M. R. Loken</name>
<affiliation wicri:level="1"><inist:fA14 i1="08"><s1>HematoLogics, Inc.</s1>
<s2>Seattle, WA</s2>
<s3>USA</s3>
<sZ>9 aut.</sZ>
<sZ>18 aut.</sZ>
<sZ>36 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Seattle, WA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Malcovati, L" sort="Malcovati, L" uniqKey="Malcovati L" first="L." last="Malcovati">L. Malcovati</name>
<affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Department of Hematology and Oncology, Fondazione IRCCS Policlinico San Matteo and University of Pavia</s1>
<s2>Pavia</s2>
<s3>ITA</s3>
<sZ>10 aut.</sZ>
<sZ>19 aut.</sZ>
</inist:fA14>
<country>Italie</country>
<wicri:noRegion>Pavia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Te Marvelde, J G" sort="Te Marvelde, J G" uniqKey="Te Marvelde J" first="J. G." last="Te Marvelde">J. G. Te Marvelde</name>
<affiliation wicri:level="3"><inist:fA14 i1="14"><s1>Department of Immunology, Erasmus MC</s1>
<s2>Rotterdam</s2>
<s3>NLD</s3>
<sZ>20 aut.</sZ>
<sZ>34 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Rotterdam</settlement>
<region nuts="2" type="province">Hollande-Méridionale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Matarraz, S" sort="Matarraz, S" uniqKey="Matarraz S" first="S." last="Matarraz">S. Matarraz</name>
<affiliation wicri:level="1"><inist:fA14 i1="15"><s1>Servicio Central de Citometria, Centro de Investigación del Cancer, Instituto de Biologia Celular y Molecular del Cancer (CSIC/USAL) and Department of Medicine, Universidad de Salamanca</s1>
<s2>Salamanca</s2>
<s3>ESP</s3>
<sZ>21 aut.</sZ>
<sZ>26 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>Salamanca</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Milne, T" sort="Milne, T" uniqKey="Milne T" first="T." last="Milne">T. Milne</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Haematology, King's College Hospital</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>2 aut.</sZ>
<sZ>17 aut.</sZ>
<sZ>22 aut.</sZ>
<sZ>24 aut.</sZ>
<sZ>31 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Moshaver, B" sort="Moshaver, B" uniqKey="Moshaver B" first="B." last="Moshaver">B. Moshaver</name>
<affiliation wicri:level="3"><inist:fA14 i1="16"><s1>Department of Hematology, St Radboud University Medical Center</s1>
<s2>Nijmegen</s2>
<s3>NLD</s3>
<sZ>23 aut.</sZ>
<sZ>35 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Nimègue</settlement>
<region type="province" nuts="2">Gueldre</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mufti, G J" sort="Mufti, G J" uniqKey="Mufti G" first="G. J." last="Mufti">G. J. Mufti</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Haematology, King's College Hospital</s1>
<s2>London</s2>
<s3>GBR</s3>
<sZ>2 aut.</sZ>
<sZ>17 aut.</sZ>
<sZ>22 aut.</sZ>
<sZ>24 aut.</sZ>
<sZ>31 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
<placeName><settlement type="city">Londres</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Grand Londres</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ogata, K" sort="Ogata, K" uniqKey="Ogata K" first="K." last="Ogata">K. Ogata</name>
<affiliation wicri:level="3"><inist:fA14 i1="17"><s1>Division of Hematology, Department of Medicine, Nippon Medical School</s1>
<s2>Tokyo</s2>
<s3>JPN</s3>
<sZ>25 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Orfao, A" sort="Orfao, A" uniqKey="Orfao A" first="A." last="Orfao">A. Orfao</name>
<affiliation wicri:level="1"><inist:fA14 i1="15"><s1>Servicio Central de Citometria, Centro de Investigación del Cancer, Instituto de Biologia Celular y Molecular del Cancer (CSIC/USAL) and Department of Medicine, Universidad de Salamanca</s1>
<s2>Salamanca</s2>
<s3>ESP</s3>
<sZ>21 aut.</sZ>
<sZ>26 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>Salamanca</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Porwit, A" sort="Porwit, A" uniqKey="Porwit A" first="A." last="Porwit">A. Porwit</name>
<affiliation wicri:level="3"><inist:fA14 i1="18"><s1>Department of Pathology, Karolinska University Hospital</s1>
<s2>Stockholm</s2>
<s3>SWE</s3>
<sZ>27 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<placeName><settlement type="city">Stockholm</settlement>
<region nuts="2">Svealand</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Psarra, K" sort="Psarra, K" uniqKey="Psarra K" first="K." last="Psarra">K. Psarra</name>
<affiliation wicri:level="3"><inist:fA14 i1="19"><s1>Department of Immunology-Histocompatibility, Evangelismos Hospital</s1>
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<s3>GRC</s3>
<sZ>28 aut.</sZ>
</inist:fA14>
<country>Grèce</country>
<placeName><settlement type="city">Athènes</settlement>
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</affiliation>
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<author><name sortKey="Richards, S J" sort="Richards, S J" uniqKey="Richards S" first="S. J." last="Richards">S. J. Richards</name>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Haematology, HMDS, St James's University Hospital</s1>
<s2>Leeds</s2>
<s3>GBR</s3>
<sZ>8 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
<country>Royaume-Uni</country>
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</author>
<author><name sortKey="Subira, D" sort="Subira, D" uniqKey="Subira D" first="D." last="Subira">D. Subira</name>
<affiliation wicri:level="1"><inist:fA14 i1="20"><s1>Department of Hematology, Hospital Universitario de Guadalajara</s1>
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<sZ>30 aut.</sZ>
</inist:fA14>
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<wicri:noRegion>Guadalajara</wicri:noRegion>
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<author><name sortKey="Tindell, V" sort="Tindell, V" uniqKey="Tindell V" first="V." last="Tindell">V. Tindell</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Haematology, King's College Hospital</s1>
<s2>London</s2>
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<sZ>2 aut.</sZ>
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<sZ>22 aut.</sZ>
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<author><name sortKey="Vallespi, T" sort="Vallespi, T" uniqKey="Vallespi T" first="T." last="Vallespi">T. Vallespi</name>
<affiliation wicri:level="3"><inist:fA14 i1="21"><s1>Department of Hematology, Hospital Universitario</s1>
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<s3>ESP</s3>
<sZ>32 aut.</sZ>
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<author><name sortKey="Valent, P" sort="Valent, P" uniqKey="Valent P" first="P." last="Valent">P. Valent</name>
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<s3>AUT</s3>
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<country>Autriche</country>
<placeName><settlement type="city">Vienne (Autriche)</settlement>
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<author><name sortKey="Der Velden, Vhj Van" sort="Der Velden, Vhj Van" uniqKey="Der Velden V" first="Vhj Van" last="Der Velden">Vhj Van Der Velden</name>
<affiliation wicri:level="3"><inist:fA14 i1="14"><s1>Department of Immunology, Erasmus MC</s1>
<s2>Rotterdam</s2>
<s3>NLD</s3>
<sZ>20 aut.</sZ>
<sZ>34 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
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</author>
<author><name sortKey="De Witte, T M" sort="De Witte, T M" uniqKey="De Witte T" first="T. M." last="De Witte">T. M. De Witte</name>
<affiliation wicri:level="3"><inist:fA14 i1="16"><s1>Department of Hematology, St Radboud University Medical Center</s1>
<s2>Nijmegen</s2>
<s3>NLD</s3>
<sZ>23 aut.</sZ>
<sZ>35 aut.</sZ>
</inist:fA14>
<country>Pays-Bas</country>
<placeName><settlement type="city">Nimègue</settlement>
<region type="province" nuts="2">Gueldre</region>
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</affiliation>
</author>
<author><name sortKey="Wells, D A" sort="Wells, D A" uniqKey="Wells D" first="D. A." last="Wells">D. A. Wells</name>
<affiliation wicri:level="1"><inist:fA14 i1="08"><s1>HematoLogics, Inc.</s1>
<s2>Seattle, WA</s2>
<s3>USA</s3>
<sZ>9 aut.</sZ>
<sZ>18 aut.</sZ>
<sZ>36 aut.</sZ>
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<country>États-Unis</country>
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</affiliation>
</author>
<author><name sortKey="Zettl, F" sort="Zettl, F" uniqKey="Zettl F" first="F." last="Zettl">F. Zettl</name>
<affiliation wicri:level="3"><inist:fA14 i1="12"><s1>Department of Hematology and Oncology, Georg-August-University</s1>
<s2>Göttingen</s2>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
<sZ>37 aut.</sZ>
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<country>Allemagne</country>
<placeName><region type="land" nuts="2">Basse-Saxe</region>
<settlement type="city">Göttingen</settlement>
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</affiliation>
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<author><name sortKey="Bene, M C" sort="Bene, M C" uniqKey="Bene M" first="M. C." last="Bene">M. C. Bene</name>
<affiliation wicri:level="4"><inist:fA14 i1="23"><s1>Faculty of Medicine and CHU, Nancy Université</s1>
<s2>Nancy</s2>
<s3>FRA</s3>
<sZ>38 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Grand Est</region>
<region type="old region">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
<settlement type="city">Nancy</settlement>
</placeName>
<orgName type="university">Nancy-Université</orgName>
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</author>
<author><name sortKey="De Loosdrecht, Aa Van" sort="De Loosdrecht, Aa Van" uniqKey="De Loosdrecht A" first="Aa Van" last="De Loosdrecht">Aa Van De Loosdrecht</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Hematology, VU University Medical Center</s1>
<s2>Amsterdam</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>39 aut.</sZ>
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<country>Pays-Bas</country>
<placeName><settlement type="city">Amsterdam</settlement>
<region nuts="2" type="province">Hollande-Septentrionale</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Leukemia</title>
<title level="j" type="abbreviated">Leukemia</title>
<idno type="ISSN">0887-6924</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Leukemia</title>
<title level="j" type="abbreviated">Leukemia</title>
<idno type="ISSN">0887-6924</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Consensus</term>
<term>Europe</term>
<term>Flow cytometry</term>
<term>Hematology</term>
<term>International</term>
<term>Myelodysplastic syndrome</term>
<term>Standardization</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Syndrome myélodysplasique</term>
<term>Normalisation</term>
<term>Cytométrie flux</term>
<term>International</term>
<term>Europe</term>
<term>Consensus</term>
<term>Hématologie</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Normalisation</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Flow cytometry (FC) is increasingly recognized as an important tool in the diagnosis and prognosis of myelodysplastic syndromes (MDS). However, validation of current assays and agreement upon the techniques are prerequisites for its widespread acceptance and application in clinical practice. Therefore, a working group was initiated (Amsterdam, 2008) to discuss and propose standards for FC in MDS. In 2009 and 2010, representatives from 23, mainly European, institutes participated in the second and third European LeukemiaNet (ELN) MDS workshops. In the present report, minimal requirements to analyze dysplasia are refined. The proposed core markers should enable a categorization of FC results in cytopenic patients as 'normal', 'suggestive of', or 'diagnostic of' MDS. An FC report should include a description of validated FC abnormalities such as aberrant marker expression on myeloid progenitors and, furthermore, dysgranulopoiesis and/or dysmonocytopoiesis, if at least two abnormalities are evidenced. The working group is dedicated to initiate further studies to establish robust diagnostic and prognostic FC panels in MDS. An ultimate goal is to refine and improve diagnosis and prognostic scoring systems. Finally, the working group stresses that FC should be part of an integrated diagnosis rather than a separate technique.</div>
</front>
</TEI>
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<li>Australie</li>
<li>Autriche</li>
<li>Espagne</li>
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<orgName><li>Nancy-Université</li>
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<tree><country name="Pays-Bas"><region name="Hollande-Septentrionale"><name sortKey="Westers, T M" sort="Westers, T M" uniqKey="Westers T" first="T. M." last="Westers">T. M. Westers</name>
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<name sortKey="Alhan, C" sort="Alhan, C" uniqKey="Alhan C" first="C." last="Alhan">C. Alhan</name>
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<name sortKey="Dr Ger, A M" sort="Dr Ger, A M" uniqKey="Dr Ger A" first="A. M." last="Dr Ger">A. M. Dr Ger</name>
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<name sortKey="Kordasti, S" sort="Kordasti, S" uniqKey="Kordasti S" first="S." last="Kordasti">S. Kordasti</name>
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<name sortKey="Malcovati, L" sort="Malcovati, L" uniqKey="Malcovati L" first="L." last="Malcovati">L. Malcovati</name>
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<country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Feuillard, J" sort="Feuillard, J" uniqKey="Feuillard J" first="J." last="Feuillard">J. Feuillard</name>
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<name sortKey="Subira, D" sort="Subira, D" uniqKey="Subira D" first="D." last="Subira">D. Subira</name>
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Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group

Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group

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